Fermentation
Microbial isolates are grown in nutrient, marine or A1 liquid medium.
Culture medium (5 L) is initially shaken at 180 rpm for 5 days at room
temperature and the microbial cells are then separated from the liquid
broth via vacuum filtration. Both the microbial cells and liquid broth
are extracted with ethyl acetate. Organic solvents are removed in
vacuo to afford crude extracts.
Large scale fermentation Crude extracts
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