Bioassay

A bioassay guided fractionation approach is employed in which the crude organic extracts are purified into various subfractions and the identities of biologically active entities are determined from various assays. A series of antimicrobial and antitumor assays are employed to determine the efficacy of the microbial isolates.

Disk diffusion assay                        Bioautography assay

                           Cytotoxicity assay